Cloning and Characterization of a Laccase Gene from Biotechnologically Important Basidiomycete Cerrena Unicolor
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چکیده
منابع مشابه
Chemical Modifications of Laccase from White-Rot Basidiomycete Cerrena unicolor
Laccases belong to the group of phenol oxidizes and constitute one of the most promising classes of enzymes for future use in various fields. For industrial and biotechnological purposes, laccases were among the first enzymes providing larger-scale applications such as removal of polyphenols or conversion of toxic compounds. The wood-degrading basidiomycete Cerrena unicolor C-139, reported in t...
متن کاملCharacterization of Cellobiose Dehydrogenase from a Biotechnologically Important Cerrena unicolor Strain
Cellobiose dehydrogenase (CDH), a secreted flavocytochrome produced by a number of wood-degrading fungi, was detected in the culture supernatant of a biotechnologically important strain of Cerrena unicolor grown in a modified cellulose-based liquid medium. The enzyme was purified as two active fractions: CuCDH-FAD (flavin domain) (1.51-fold) with recovery of 8.35 % and CuCDH (flavo-heme enzyme)...
متن کاملModulation of Cerrena unicolor laccase and manganese peroxidase production
Among seven carbon sources tested, glycerol and glucose favored the Cerrena unicolor laccase production (18.8-20.3 U/mL); in addition, glycerol ensured the highest manganese peroxidase (MnP) activity (2 U/mL). Substitution of glycerol with the ethanol production residue (EPR) gave the highest laccase (90.1 U/mL) activity, while the walnut pericarp provided the highest MnP activity (7.4 U/mL). S...
متن کاملBiochemical characterization of a novel laccase from the basidiomycete fungus Cerrena sp. WR1.
This study reports a new white-rot fungus Cerrena sp. WR1, identified based on an 18S rDNA sequence, which can secrete extracellular forms of laccase with a maximal activity reaching 202 000 U l⁻¹ in a 5-l fermenter. A laccase protein, designated Lcc3, was purified and shown to be N-linked glycosylated by PNGase F and liquid chromatography tandem mass spectrometry analyses. The respective full-...
متن کاملLaccase purified from Cerrena unicolor exerts antitumor activity against leukemic cells
Chronic lymphocytic leukemia (CLL) is the most commonly observed adult hematological malignancy in Western countries. Despite the fact that recent improvements in CLL treatment have led to an increased percentage of complete remissions, CLL remains an incurable disease. Cerrena unicolor is a novel fungal source of highly active extracellular laccase (ex-LAC) that is currently used in industry. ...
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ژورنال
عنوان ژورنال: Journal of the Faculty of Agriculture, Kyushu University
سال: 2012
ISSN: 0023-6152
DOI: 10.5109/22046